Journal: Nutrients

Article Title: Effect Assessment of Aurantio-Obtusin on Novel Human Renal Glomerular Endothelial Cells Model Using a Microfluidic Chip

doi: 10.3390/nu14214615

Figure Lengend Snippet: Design of a CellASIC-based microfluidic platform to culture glomerular endothelial cells. ( A ) CellASIC ONIX2 Microfluidic Platform. The Platform consists of a controller system including multiple control panels, a computer software interface and a fluorescent inverted microscope. ( B ) Configuration of the microfluidic chip device. The microfluidic plate consists of four independent culture units, each with a gravity flow inlet (1), four solution inlets (2–5), and a cell inlet (6). Each row of wells addresses the corresponding culture chamber. ( C ) Bright-field images showing that HRGECs grew on the microfluidic chip at different culture times (0, 1, 2, 3 d). ( D ) Fluorescence images of live/dead staining of HRGECs after 3 days of culture on the chip.

Article Snippet: The CellASIC ® ONIX2 Microfluidic Platform and the M04S microfluidic plate (Merck Millipore, Hayward, CA, USA) were used for cell culture.

Techniques: Control, Software, Inverted Microscopy, Fluorescence, Staining

Journal: Nutrients

Article Title: Effect Assessment of Aurantio-Obtusin on Novel Human Renal Glomerular Endothelial Cells Model Using a Microfluidic Chip

doi: 10.3390/nu14214615

Figure Lengend Snippet: Cytotoxic effects of AO on glomerular endothelial cells. ( A ) CCK-8 assay of HRGEC viability in 96-well plates. ( B ) CCK-8 assay of HRGECs viability on the microfluidic chip. ( C ) Morphological changes in HRGECs after exposure to AO on the microfluidic chip were observed using a bright-field microscope. ( D ) Fluorescence images of live/dead staining of HRGECs on the chip after exposure to AO. The live cells were stained green and the dead cells were stained red. ( E ) LDH leakage assay in HRGECs. ( F ) AO treatment increased the expression of pro-inflammatory cytokines IL-6 and TNF-α. ( G ) AO treatment increased the expression of cytokines TGF-β 1 and MCP-1. The data are presented as means ± standard deviation. n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The CellASIC ® ONIX2 Microfluidic Platform and the M04S microfluidic plate (Merck Millipore, Hayward, CA, USA) were used for cell culture.

Techniques: CCK-8 Assay, Microscopy, Fluorescence, Staining, Expressing, Standard Deviation